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Biblioteca (s) : |
INIA Tacuarembó; INIA Treinta y Tres. |
Fecha : |
07/06/2019 |
Actualizado : |
18/11/2021 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
IRISARRI, P.; CARDOZO, G.; TARTAGLIA, C.; REYNO, R.; GUTIÉRREZ, P.; LATTANZI, F.; REBUFFO, M.; MONZA, J. |
Afiliación : |
PILAR IRISARRI, Laboratorio de Microbiología, Departamento de Biología Vegetal, Facultad de Agronomía, UDELAR, URUGUAY; GERONIMO AGUSTIN CARDOZO CABANELAS, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CAROLINA TARTAGLIA, Laboratorio de Bioquimica, Departamento de Biología Vegetal, Facultad de Agronomía, UDELAR, Uruguay.; RAFAEL ALEJANDRO REYNO PODESTA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; PAMELA GUTIÉRREZ, Laboratorio de Bioquímica, Departamento de Biología Vegetal, Facultad de Agronomía, UDELAR, URUGUAY.; FERNANDO A. LATTANZI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MONICA IRENE REBUFFO GFELLER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JORGE MONZA, Laboratorio de Bioquímica, Departamento de Biología Vegetal, Facultad de Agronomía, UDELAR, URUGUAY. |
Título : |
Selection of competitive and efficient rhizobia strains for white clover. |
Fecha de publicación : |
2019 |
Fuente / Imprenta : |
Frontiers in Microbiology, 23 April 2019, v. 10, art. 768. DOI: https://doi.org/10.3389/fmicb.2019.00768 |
Páginas : |
11 p. |
DOI : |
10.3389/fmicb.2019.00768 |
Idioma : |
Inglés |
Notas : |
Article history: Received 28 September 2018 // Accepted 26 March 2019 // Published: 23 April 2019. Open Access Journal. Acknowledgments: We are grateful to Verónica Berriel for the N abundance determination and to Carlos Rossi for providing white clover seeds. |
Contenido : |
The practice of inoculating forage legumes with rhizobia strains is widespread. It is assumed that the inoculated strain determines the performance of the symbiosis and nitrogen fixation rates. However, native-naturalized strains can be competitive, and actual nodule occupancy is often scarcely investigated. In consequence, failures in establishment, and low productivity attributed to poor performance of the inoculant may merely reflect the absence of the inoculated strain in the nodules. This study lays out a strategy followed for selecting a Rhizobium leguminosarum sv. trifolii strain for white clover (Trifolium repens) with competitive nodule occupancy. First, the competitiveness of native-naturalized rhizobia strains selected for their efficiency to fix N2 in clover and tagged with gusA was evaluated in controlled conditions with different soils. Second, three of these experimental strains with superior nodule occupancy plus the currently recommended commercial inoculant, an introduced strain, were tested in the field in 2 years and at two sites. Plant establishment, herbage productivity, fixation of atmospheric N2 (15N natural abundance), and nodule occupancy (ERIC-PCR genomic fingerprinting) were measured. In both years and sites, nodule occupancy of the nativenaturalized experimental strains was either higher or similar to that of the commercial inoculant in both primary and secondary roots. The difference was even greater in stolon roots nodules, where nodule occupancy of the native-naturalized experimental strains was at least five times greater. The amount of N fixed per unit plant mass was consistently higher with native-naturalized experimental strains, although the proportion of N derived from atmospheric fixation was similar for all strains. Plant establishment and herbage production, as well as clover contribution in oversown native grasslands, were either similar or higher in white clover inoculated with the native-naturalized experimental strains. These results support the use of our implemented strategy for developing a competitive inoculant from native-naturalized strains. MenosThe practice of inoculating forage legumes with rhizobia strains is widespread. It is assumed that the inoculated strain determines the performance of the symbiosis and nitrogen fixation rates. However, native-naturalized strains can be competitive, and actual nodule occupancy is often scarcely investigated. In consequence, failures in establishment, and low productivity attributed to poor performance of the inoculant may merely reflect the absence of the inoculated strain in the nodules. This study lays out a strategy followed for selecting a Rhizobium leguminosarum sv. trifolii strain for white clover (Trifolium repens) with competitive nodule occupancy. First, the competitiveness of native-naturalized rhizobia strains selected for their efficiency to fix N2 in clover and tagged with gusA was evaluated in controlled conditions with different soils. Second, three of these experimental strains with superior nodule occupancy plus the currently recommended commercial inoculant, an introduced strain, were tested in the field in 2 years and at two sites. Plant establishment, herbage productivity, fixation of atmospheric N2 (15N natural abundance), and nodule occupancy (ERIC-PCR genomic fingerprinting) were measured. In both years and sites, nodule occupancy of the nativenaturalized experimental strains was either higher or similar to that of the commercial inoculant in both primary and secondary roots. The difference was even greater in stolon roots nodules, where nodule occupan... Presentar Todo |
Palabras claves : |
BIOLOGICAL NITROGEN FIXATION; INOCULANT; NATIVE-NATURALIZED RHIZOBIA STRAINS; NODULE OCCUPANCY; R. LEGUMINOSARUM SV. TRIFOLII; WHITE CLOVER. |
Thesagro : |
FIJACION BIOLOGICA DEL NITRÓGENO; NODULACION; TREBOL BLANCO; TRIFOLIUM REPENS. |
Asunto categoría : |
F60 Fisiología y bioquímica de la planta |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/12797/1/Cardozo-Frontier-Micro-2019.pdf
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Marc : |
LEADER 03468naa a2200361 a 4500 001 1059873 005 2021-11-18 008 2019 bl uuuu u00u1 u #d 024 7 $a10.3389/fmicb.2019.00768$2DOI 100 1 $aIRISARRI, P. 245 $aSelection of competitive and efficient rhizobia strains for white clover.$h[electronic resource] 260 $c2019 300 $a11 p. 500 $aArticle history: Received 28 September 2018 // Accepted 26 March 2019 // Published: 23 April 2019. Open Access Journal. Acknowledgments: We are grateful to Verónica Berriel for the N abundance determination and to Carlos Rossi for providing white clover seeds. 520 $aThe practice of inoculating forage legumes with rhizobia strains is widespread. It is assumed that the inoculated strain determines the performance of the symbiosis and nitrogen fixation rates. However, native-naturalized strains can be competitive, and actual nodule occupancy is often scarcely investigated. In consequence, failures in establishment, and low productivity attributed to poor performance of the inoculant may merely reflect the absence of the inoculated strain in the nodules. This study lays out a strategy followed for selecting a Rhizobium leguminosarum sv. trifolii strain for white clover (Trifolium repens) with competitive nodule occupancy. First, the competitiveness of native-naturalized rhizobia strains selected for their efficiency to fix N2 in clover and tagged with gusA was evaluated in controlled conditions with different soils. Second, three of these experimental strains with superior nodule occupancy plus the currently recommended commercial inoculant, an introduced strain, were tested in the field in 2 years and at two sites. Plant establishment, herbage productivity, fixation of atmospheric N2 (15N natural abundance), and nodule occupancy (ERIC-PCR genomic fingerprinting) were measured. In both years and sites, nodule occupancy of the nativenaturalized experimental strains was either higher or similar to that of the commercial inoculant in both primary and secondary roots. The difference was even greater in stolon roots nodules, where nodule occupancy of the native-naturalized experimental strains was at least five times greater. The amount of N fixed per unit plant mass was consistently higher with native-naturalized experimental strains, although the proportion of N derived from atmospheric fixation was similar for all strains. Plant establishment and herbage production, as well as clover contribution in oversown native grasslands, were either similar or higher in white clover inoculated with the native-naturalized experimental strains. These results support the use of our implemented strategy for developing a competitive inoculant from native-naturalized strains. 650 $aFIJACION BIOLOGICA DEL NITRÓGENO 650 $aNODULACION 650 $aTREBOL BLANCO 650 $aTRIFOLIUM REPENS 653 $aBIOLOGICAL NITROGEN FIXATION 653 $aINOCULANT 653 $aNATIVE-NATURALIZED RHIZOBIA STRAINS 653 $aNODULE OCCUPANCY 653 $aR. LEGUMINOSARUM SV. TRIFOLII 653 $aWHITE CLOVER 700 1 $aCARDOZO, G. 700 1 $aTARTAGLIA, C. 700 1 $aREYNO, R. 700 1 $aGUTIÉRREZ, P. 700 1 $aLATTANZI, F. 700 1 $aREBUFFO, M. 700 1 $aMONZA, J. 773 $tFrontiers in Microbiology, 23 April 2019$gv. 10, art. 768. DOI: https://doi.org/10.3389/fmicb.2019.00768
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INIA Tacuarembó (TBO) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha actual : |
12/04/2017 |
Actualizado : |
12/04/2017 |
Tipo de producción científica : |
Presentaciones Orales |
Autor : |
VIGNALE, B.; CABRERA, D.; NEBEL, J.P.; GONZÁLEZ, S.; RODRIGUEZ, P.; ZOPPOLO, R.; LÓPEZ, H.; FAGÚNDEZ, V. |
Afiliación : |
CARLOS DANILO CABRERA BOLOGNA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; PABLO ANDRES RODRIGUEZ BRUNO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ROBERTO JOSE ZOPPOLO GOLDSCHMIDT, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Selección de frutas nativas. Avances. [Presentación oral]. |
Fecha de publicación : |
2015 |
Fuente / Imprenta : |
In: UNIVERSIDAD DE LA REPÚBLICA (UDELAR)/ FACULTAD DE AGRONOMÍA; MGAP/ DGF (DIRECCIÓN GENERAL FORESTAL); INIA, PROGRAMA NACIONAL PRODUCCIÓN FRUTÍCOLA. 7° Encuentro Nacional sobre Frutos Nativos. Colonia: Estación Experimental "Alberto Boerger", INIA La Estanzuela, 9 y 10 de abril de 2015. |
Idioma : |
Español |
Palabras claves : |
AGUAÍ; ARAZÁ AMARILLO; ARAZÁ ROJO; BAPORETÍ; CEREZA DEL MONTE; QUEBRACHO FLOJO; UBAJAY. |
Thesagro : |
ARAZÁ; FRUTOS NATIVOS; GUAVIYU; GUAYABO DEL PAÍS; PITANGA. |
Asunto categoría : |
-- |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/6666/1/Seleccion-de-frutas-nativas-BVignale-2015.pdf
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Marc : |
LEADER 01103nam a2200325 a 4500 001 1057035 005 2017-04-12 008 2015 bl uuuu u00u1 u #d 100 1 $aVIGNALE, B. 245 $aSelección de frutas nativas. Avances. [Presentación oral].$h[electronic resource] 260 $aIn: UNIVERSIDAD DE LA REPÚBLICA (UDELAR)/ FACULTAD DE AGRONOMÍA; MGAP/ DGF (DIRECCIÓN GENERAL FORESTAL); INIA, PROGRAMA NACIONAL PRODUCCIÓN FRUTÍCOLA. 7° Encuentro Nacional sobre Frutos Nativos. Colonia: Estación Experimental "Alberto Boerger", INIA La Estanzuela, 9 y 10 de abril de 2015.$c2015 650 $aARAZÁ 650 $aFRUTOS NATIVOS 650 $aGUAVIYU 650 $aGUAYABO DEL PAÍS 650 $aPITANGA 653 $aAGUAÍ 653 $aARAZÁ AMARILLO 653 $aARAZÁ ROJO 653 $aBAPORETÍ 653 $aCEREZA DEL MONTE 653 $aQUEBRACHO FLOJO 653 $aUBAJAY 700 1 $aCABRERA, D. 700 1 $aNEBEL, J.P. 700 1 $aGONZÁLEZ, S. 700 1 $aRODRIGUEZ, P. 700 1 $aZOPPOLO, R. 700 1 $aLÓPEZ, H. 700 1 $aFAGÚNDEZ, V.
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INIA Las Brujas (LB) |
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